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Journal / Biochemistry / 用核磁共振 (nmr) 和微尺度热能 (mst)…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
用核磁共振 (nmr) 和微尺度热能 (mst) 测量球状和丝状蛋白的相互作用
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Journal (Biochemistry)
A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
Journal (Biochemistry)
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
 
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用核磁共振 (nmr) 和微尺度热能 (mst) 测量球状和丝状蛋白的相互作用

Article DOI: 10.3791/58537-v • 10:28 min • November 2nd, 2018
November 2nd, 2018 •
1, 1, 1, 1
1Department of Biochemistry, University of Alberta

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在这里, 我们提出了一个协议, 生产和纯化的蛋白质标记与稳定同位素, 以及随后的表征蛋白质相互作用使用核磁共振 (nmr) 光谱和微尺度热像仪 (mst) 实验。

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生物化学 第141期 细胞粘附酶体 去角质 丝状蛋白 中间丝 微尺度热积 核磁共振光谱 蛋白质相互作用 vimentin
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