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A Micro-agar Salt Bridge Electrode for Analyzing the Proton Turnover Rate of Recombinant Membrane Proteins
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Chapters
- 00:04Title
- 00:44Preparation of the Micro-agar Salt Bridge Electrode
- 05:26Measurement of the Electrical Parameters of the Membrane Reconstituted with Recombinant Protein
- 06:23Results: Comparison of Membrane Measurements Made with AgCl and Salt Bridge Electrodes
- 07:41Conclusion
In electrophysiological measurements, the presence of a diffusion potential disturbs the precise measurement of the reverse potential by altering the electrode potential. Using a micro-agar salt bridge, the impact of the diffusion potential is minimized, which allows a more precise measurement of substrate turnover numbers of reconstituted recombinant membrane proteins.
