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Immunology and Infection
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Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

Establishment and Analysis of Tumor Slice Explants As a Prerequisite for Diagnostic Testing

DOI:
10.3791/58569-v

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11:00 min

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November 29, 2018

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, , , , ,
1Institute for Molecular Medicine Finland (FIMM), HiLIFE,University of Helsinki

Chapters

  • 00:04Title
  • 00:42Collection of Tumor-bearing Lungs
  • 02:08Generation and Cultivation of Precision-cut Lung Tumor Slices, and Treatment with Inhibitors
  • 05:58Fixation and Processing of the Tissue Slices
  • 07:27Processing and Analysis of Formalin-fixed and Paraffin-embedded (FFPE) Tissues
  • 08:33Results: Assessment of Viability, Specific Marker Expression, and Targeted Drug Treatment on Adenocarcinoma Tissue Slices
  • 10:26Conclusion

Summary

Automatic Translation

Automatic Translation

We provide a method for the generation, cultivation and systematic analysis of organotypic slices derived from murine lung tumors. We also describe how to optimize for slice thickness, and how to select drug concentrations to treat tumor slices.

Tags

Keywords: Tumor Slice ExplantsPreclinical ResearchDrug Response StudiesMurine Lung TumorsVibratomeHank’s Balanced Salt SolutionEuthanized MouseLungHeartCyanoacrylate Adhesive

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