/

Immunology and Infection

/

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

JoVE Journal
Immunology and Infection
Author Produced

This content is Free Access.

JoVE Journal Immunology and Infection

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

DOI:

10.3791/58574-v

•

14:54 min

•

January 27, 2019

•

Byron Brook*¹, Nelly Amenyogbe*¹, Rym Ben-Othman, Bing Cai, Danny Harbeson, Freddy Francis, Aaron C. Liu, Natallia Varankovich, James Wynn⁴, Tobias R. Kollmann²

¹Department of Experimental Medicine,University of British Columbia, ²Department of Pediatrics, Division of Infectious Diseases,University of British Columbia, ³Department of Pediatrics, College of Medicine,University of Florida, ⁴Department of Pathology, Immunology, and Laboratory Medicine,University of Florida

Chapters

00:00Title
00:33Preparation of Frozen Cecal Slurry Stock from Adult Mouse Donors
04:42Challenging Newborn Mice with a Weight-adjusted Dose of Cecal Slurry
08:00Monitoring Mice and Determining Humane Endpoint
10:34Representative Results
14:28Conclusion

Summary

Automatic Translation

This protocol provides the necessary steps to establish and evaluate neonatal sepsis in 7-day-old mice.

Tags

Keywords: Neonatal Polymicrobial Sepsis Cecal Slurry Model Standardized Procedure Data-driven Approach Moribund Pups Frozen Cecal Slurry Stock Cecal Contents Dissection Sterile Tools Biological Safety Cabinet

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Colon Ascendens Stent Peritonitis (CASP) – a Standardized Model for Polymicrobial Abdominal Sepsis

Cecal Ligation and Puncture-induced Sepsis as a Model To Study Autophagy in Mice

A Mouse Model for Pathogen-induced Chronic Inflammation at Local and Systemic Sites

Non-Invasive Model of Neuropathogenic Escherichia coli Infection in the Neonatal Rat

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Influenza A Virus Studies in a Mouse Model of Infection

Effects of Exposure of Formaldehyde to a Rat Model of Atopic Dermatitis Induced by Neonatal Capsaicin Treatment

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Read Article