Humane Endpoint Monitoring
Humane Endpoint Monitoring - Related Videos
01:19
Precipitation Titration: Endpoint Detection Methods
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2025
In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3. In the Volhard method, a standard excess of AgNO3 is first added to the...
09:55
Monitoring Acupuncture Effects on Human Brain by fMRI
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Cited by 82 •
2010
FMRI and physiological monitoring is used to study the effects of Acupuncture on the central and peripheral nervous systems. Acupuncture mobilizes a limbic-paralimbic-neocortical network, with great overlap with the default mode network, to modulate neurological activity, possibly related to its autonomic effect in the peripheral nervous system.
06:28
Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
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Cited by 9 •
2015
The human lactoferrin (hLF) is a component of the immune system. In this study, immunofluorescence assays are used to demonstrate both the hepatocellular uptake of hLF and a qualitative reduction in the hepatitis C virus replication upon treatment with hLF.
07:51
A Novel Approach to Monitoring Graft Neovascularization in the Human Gingiva
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Cited by 7 •
2019
This study introduces a protocol for measuring microcirculation in human oral mucosa by laser speckle contrast imaging. The monitoring of wound healing after vestibuloplasty combined with a xenogenic collagen graft is presented on a clinical case.
15:09
The Use of Primary Human Fibroblasts for Monitoring Mitochondrial Phenotypes in the Field of Parkinson's Disease
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Cited by 22 •
2012
Fibroblasts from patients carrying mutations in Parkinson's disease-causing genes represent an easily accessible ex vivo model to study disease-associated phenotypes. Live cell imaging gives the opportunity to study morphological and functional parameters in living cells. Here we describe the preparation of human fibroblasts and subsequent monitoring of mitochondrial phenotypes.
FAQs
Predefined thresholds make decisions more consistent by linking specific findings, such as weight loss, altered respiration, or reduced activity, to actions established in the study protocol. They also reduce reliance on individual judgment during stressful cases. In infection and immunology studies, this structure supports timely intervention while helping ensure that comparable disease severity leads to comparable welfare decisions.
A sequence of observations can reveal progressive deterioration that a single examination may miss. Changes in body weight, body condition, posture, activity, temperature, or disease-specific signs become more informative when evaluated over time. Monitoring trends therefore helps distinguish a transient finding from worsening illness and supports earlier, more consistent responses under the approved protocol.
Timely decisions can limit complications associated with advanced illness, which may otherwise introduce additional physiological changes unrelated to the primary study question. By reducing severe deterioration and applying consistent criteria, monitoring can improve animal welfare while also producing more reliable data. Its value is therefore both ethical and scientific, particularly when disease progression could alter experimental outcomes.
Personnel should record both general condition and findings relevant to the disease model. Useful observations include body weight, body condition, activity, posture, respiration, temperature, and disease-specific clinical signs. Recording these measures systematically allows the study team to identify meaningful changes, compare observations with protocol thresholds, and document the basis for intervention, supportive care, or euthanasia.
The study protocol establishes scheduled observations and specifies the measures to assess at those time points. Trained personnel then document findings consistently and compare them with predefined thresholds rather than waiting for severe illness to become obvious. When observations indicate worsening disease, the protocol guides the next action, which may include intervention, supportive care, or euthanasia.
Disease-specific signs are important when general measures alone do not fully describe the progression of an infection or immunological challenge. They provide additional context alongside weight, activity, respiration, temperature, and body condition. Including them in the monitoring plan helps the team recognize clinically meaningful deterioration and apply endpoint criteria that are appropriate to the particular study.