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Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia
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Neuroscience
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JoVE Journal Neuroscience
Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

Cell-based Assay to Study Antibody-mediated Tau Clearance by Microglia

DOI:
10.3791/58576-v

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07:18 min

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November 09, 2018

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, , , , ,
1Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Belgium, 2Department of Hematopoiesis,Sanquin Research and Landsteiner Laboratory, The Netherlands, 3Janssen Prevention Center,Janssen Pharmaceutical Company of Johnson & Johnson, The Netherlands, 4Neuroscience Discovery,Janssen Pharmaceutical Company of Johnson & Johnson, Pennsylvania

Chapters

  • 00:04Title
  • 00:37Uptake Assay with Fluorescence-activated Cell Sorting (FACS) Read-out
  • 04:33FACS Analysis
  • 05:41Results: Antibody-mediated Increase of Tau Uptake in BV-2 Cells
  • 06:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a cell-based assay to quantitatively assess tau uptake by microglia with the aim of creating an investigational tool to better characterize the mechanisms of action of anti-tau antibodies.

Tags

Keywords: Cell-based AssayAntibody-mediated Tau ClearanceMicrogliaAlzheimer’s DiseasePH Dye-Tau AggregatesAnti-Tau AntibodiesBV-2 CellsCell CultureTrypsin EDTACentrifugation

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