Journal
/
Immunology and Infection
/
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

DOI:
10.3791/58595-v

•

08:13 min

•

January 20, 2019

•

, , , , , , , ,
1Inovio Pharmaceuticals, Inc, 2Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Chapters

  • 00:04Title
  • 00:58Preparation of ELISpot-plates and Cell-stimulation
  • 02:09Blood Sample Processing
  • 03:44Plate Preparation and Cell-plating
  • 04:47Detection of Interferon-gamma Positive Spots
  • 06:34Results: ELISpot Assay Used to Characterize T-cell Responses in Guinea Pigs
  • 07:15Conclusion

Summary

Automatic Translation

Automatic Translation

The development of the interferon-gamma ELISpot assay for guinea pig PBMCs allows the characterization of T-cell responses in this highly relevant model for studying infectious diseases. We have applied the assay to measure T cell responses associated with intradermal delivery of DNA vaccines.

Tags

Keywords: ELISpot AssayT Cell ResponsesGuinea Pig ModelVaccinationImmunotherapeuticsHumoral ImmunityPeripheral BloodKineticsCapture Anti-guinea Pig Interferon Gamma Antibody VE4Density Gradient MediumPBMCs

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58595?article_type=v

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
check_url/58595?article_type=v

Retroviral Transduction of T-cell Receptors in Mouse T-cells
check_url/58595?article_type=v

Generation of Multivirus-specific T Cells to Prevent/treat Viral Infections after Allogeneic Hematopoietic Stem Cell Transplant
check_url/58595?article_type=v

In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
check_url/58595?article_type=v

Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
check_url/58595?article_type=v

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
check_url/58595?article_type=v

Transplantation of Tail Skin to Study Allogeneic CD4 T Cell Responses in Mice
check_url/58595?article_type=v

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
check_url/58595?article_type=v

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
check_url/58595?article_type=v

In Vivo Assay for Detection of Antigen-specific T-cell Cytolytic Function Using a Vaccination Model

Read Article