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JoVE Journal
Genetics

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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
 

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Article doi: 10.3791/58627
February 1st, 2019

Summary February 1st, 2019

SINEUPs are synthetic antisense non-coding RNAs, which contain a binding domain (BD) and an effector domain (ED) and up-regulate translation of target mRNA. Here, we describe detection methods for SINEUPs in cultured cell lines, analysis of their translation-promoting activity by Western-blot and a semi-automated high throughput imaging system.

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