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Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

Establishment of the Dual Humanized TK-NOG Mouse Model for HIV-associated Liver Pathogenesis

DOI:
10.3791/58645-v

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00:10 min

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September 11, 2019

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, , , ,
1Department of Pharmacology and Experimental Neuroscience,University of Nebraska Medical Center, 2Department of Pathology and Microbiology,University of Nebraska Medical Center

Chapters

  • 00:04Title
  • 00:47Processing of Umbilical Cord Blood and the Isolation of Human HSPCs
  • 04:25Preparation of Human Hepatocytes for Transplantation
  • 05:10Preparation for the Surgery and Instrasplenic Transplantation of Human HSPCs and Hepatocytes in Mice
  • 06:50HIV Infection of TK-NOG Mice and Its Effect on the Human Liver and Immune System
  • 07:48Results: Analysis of the Dual Humanized TK-NOG Mouse Model
  • 09:25Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol provides a reliable method to establish humanized mice with both human immune system and liver cells. Dual reconstituted immunodeficient mice achieved via intrasplenic injection of human hepatocytes and CD34+ hematopoietic stem cells are susceptible to human immunodeficiency virus-1 infection and recapitulate liver damage as observed in HIV-infected patients.

Tags

Keywords: HIVLiver PathogenesisHumanized Mouse ModelHematopoietic Stem CellsHepatocytesUmbilical Cord BloodCD34 Positive CellsCell SeparationMagnetic Activated Cell Sorting (MACS)Cell CountingCell CultureTransplantation

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