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Localization of the Locus Coeruleus in the Mouse Brain
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Localization of the Locus Coeruleus in the Mouse Brain

Article DOI: 10.3791/58652-v • 07:44 min • March 7th, 2019
March 7th, 2019 •
1, 2, 3, 1, 1, 4, 1
1Department of Physiology, Johns Hopkins University, School of Medicine, 2Department of Neuroscience, Johns Hopkins University, 3Department of Molecular and Medical Genetics, OHSU, 4X-ray science division, Advanced Photon Source, Argonne National Laboratory

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Summary
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The locus coeruleus is a small cluster of neurons involved in a variety of physiological processes. Here, we describe a protocol to prepare mouse brain sections for studies of proteins and metals in this nucleus.

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Localization Locus Coeruleus Mouse Brain Neurological Disorders Neuropsychiatric Disorders Parkinson Disease Alzheimer Disease Depression Bipolar Disorder Anxiety Function Dysfunction Mouse Brain Sectioning Coronal Sections Sagittal Sections Small Size Protocol Freshly Isolated Brain Anesthetized Mouse Perfused Mouse 4%PFA Solution 30% Sucrose Solution Embedding Mold Optimal Cutting Temperature Compound Minus 80 Degree Celsius Freezer Cryostat
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