Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / How to Stabilize Protein: Stability Screens for…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
How to Stabilize Protein
Extracellular Vesicle (EV) Isolation from Human Urine Samples Using the EVtrap Approach (Video) | JoVE
Journal (Biochemistry)
Extracellular Vesicle (EV) Isolation from Human Urine Samples Using the EVtrap Approach (Video) | JoVE
A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development (Video) | JoVE
Journal (Biochemistry)
A Bicelle Crystallization Setup for ABC Transporter Membrane Proteins to Advance Drug Development (Video) | JoVE
 

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Article DOI: 10.3791/58666-v • 07:22 min • February 11th, 2019
February 11th, 2019 •
1, 1, 2, 1,2
1Department of Biosciences, Durham University, 2Department of Chemistry, Durham University

Chapters

Summary
Automatic Translation

A protocol is presented to rapidly test the thermal stability of proteins in a variety of conditions through thermal shift assays and nano differential scanning fluorimetry. Buffer systems, salts and additives, together comprising three unique stability screens, are assayed with proteins to identify suitable buffers for functional and structural studies.

Tags

Stabilize Protein Stability Screens Thermal Shift Assay Nano Differential Scanning Fluorimetry Protein Chemistry Drug Discovery Thermostability Mutations Ligands Advantages Fast Standard Laboratory Medium To High Throughput Protein Sample Multichannel Pipette Buffer System SYPRO Orange Dye 96 Well Plate Centrifuge Storage TSA Experiment RTPCR System
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter