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JoVE Journal
Biochemistry

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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
 

Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

Article DOI: 10.3791/58669-v
April 21st, 2019

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Summary April 21st, 2019

Here, we describe a detailed protocol outlining a new fluorescent staining technique for total protein detection in polyacrylamide gels. The protocol utilizes a silver ion-specific fluorescence turn-on probe, which detects Ag+-protein complexes, and eliminates certain limitations of traditional chromogenic silver stains.

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