A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Ming Wai Lau Centre for Reparative Medicine,Karolinska Institutet, 2Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute of Molecular Functional Materials, State Key Laboratory of Neuroscience, Division of Biomedical Engineering, and Division of Life Science,Hong Kong University of Science and Technology
Chapters
- 00:04Title
- 00:36Preparation of the Gel
- 01:08Fixation of the Gel
- 01:40Preparation of the AgNO3 Solution and Silver Impregnation of the Gel
- 02:41Fluorogenic Development of the Gel
- 04:05Destaining and Imaging
- 04:34Results: Comparison of the Fluorescent Silver Staining to Other Staining Methods
- 05:56Conclusion
Aquí, describimos un protocolo detallado que un fluorescente nuevo coloración técnica para la detección de la proteína total en geles de poliacrilamida. El protocolo utiliza una sonda de encendido plata de iones específicos de fluorescencia que detecta Ag+-complejos de proteínas y elimina ciertas limitaciones de manchas plata cromogénicos tradicionales.
