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Journal / Genetics / Separating Bacteria by Capsule Amount Using a…
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Genetics

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Separating Bacteria by Capsule Amount Using a Discontinuous Density Gradient

Article DOI: 10.3791/58679-v • 05:52 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1, 1, 1,2
1Wellcome Sanger Institute, Wellcome Genome Campus, 2Department of Medicine, University of Cambridge

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We demonstrate the use of discontinuous density gradients to separate bacterial populations based on capsule production. This method is used to compare capsule amount between cultures, isolate mutants with a specific capsule phenotype, or to identify capsule regulators. Described here is the optimization and running of the assay.

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Bacteria Separation Capsule Amount Discontinuous Density Gradient Capsule Regulation Virulence Genes Rapid Comparisons Bacterial Species Pouring Gradients Adding Cells Visual Demonstration Sterile Loop Broth Culture Centrifugation Resuspension Density Gradient Dilutions
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