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JoVE Journal
Developmental Biology

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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells
 

RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Article doi: 10.3791/58687
November 26th, 2018

Summary November 26th, 2018

Here we describe a clinically relevant, high-efficiency, feeder-free method to reprogram human primary fibroblasts into induced pluripotent stem cells using modified mRNAs encoding reprogramming factors and mature microRNA-367/302 mimics. Also included are methods to assess reprogramming efficiency, expand clonal iPSC colonies, and confirm expression of the pluripotency marker TRA-1-60.

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