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JoVE Journal
Biology

This content is Open Access.

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
 

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

Article doi: 10.3791/58709
January 10th, 2019 Usage Statistics

Summary January 10th, 2019

This protocol describes the general processes and quality control checks necessary for preparing healthy adult mammalian single cells for droplet-based, high throughput single cell RNA-Seq preparations. Sequencing parameters, read alignment, and downstream single-cell bioinformatic analysis are also provided.

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