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Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis
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Immunology and Infection
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JoVE Journal Immunology and Infection
Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis

Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis

DOI:
10.3791/58751-v

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12:35 min

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January 04, 2019

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, , , ,
1Anesthesiology Department,The Second Hospital of Dalian Medical University, 2Scientific Research Center,The Second Hospital of Dalian Medical University

Chapters

  • 00:00Title
  • 01:40Construct the pET-SUMO-EGFP Plasmid and Induce EGFP-expression
  • 04:28Mouse Peritoneal Macrophage Isolation and Primary Culture
  • 06:57Macrophage Phagocytosis Assay using Flourescence Microscopy
  • 08:28Macrophage Phagocytosis Assay using Flow Cytometer
  • 11:05Results
  • 11:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to assess mouse peritoneal macrophage phagocytosis using enhanced green fluorescence protein-expressing Escherichia coli.

Tags

Keywords: EGFP-expressing Escherichia ColiPhagocytosisMacrophagesPeritoneal MacrophagesInnate Immune FunctionAgingFluorescence MicroscopyFlow CytometryTA CloningPET SUMO VectorBL21 Competent CellsKanamycin

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