Using Enhanced Green Fluorescence Protein-expressing Escherichia Coli to Assess Mouse Peritoneal Macrophage Phagocytosis

This protocol demonstrated a simple and producible method to assess phagocytosis ability of macrophages using EGFP-expressing Escherichia coli. Hello, my name is Jun-Yu from The Second Hospital of Dalian Medical University. Today we are going to show you an easy and visualize the way to assess macrophage phagocytosis which can be easily done within two hours.

This method was widely used in the study of the innate immune function. It is believed that innate immune function remained intact in the elderly. So today we are going to isolate the peritoneal macrophages from the aged and young mice and to see the phagocytic ability of these two groups.