Name: Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy (Video) | JoVE Journal
Uploaded: 2019-01-12T15:00:00.000+00:00
Duration: 9 min 18 s
Description: Learn motor function assessment for Duchenne muscular dystrophy using standardized clinical outcome measures. This method evaluates ambulant children through scored tasks and correlates results with quantitative muscle MRI findings.

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5 minutes

We would like to thank Lars Hintermann for taking part in the demonstration of the motor function measure and timed function tests.

Before recruitment, the study was approved by the local ethics committee [Ethics Committee of the two Basel Cantons (EKBB 63/13)] and the Swiss Drug Agency (Swissmedic 2013DR3151) and registered under ClinicalTrials.gov (NCT01995032).
1. Clinical Assessment of Muscle Function
<ol>
	<li>Motor function measure (MFM)
	<ol>
		<li>Have the patient perform each of the following tasks and score them as shown in Table 1. 
		Note: The MFM contains 32 independent items that must be performed in the given order to avoid unnecessarily repositioning and exhaustion of the patient5. See Table 1 for a detailed description of each task and scoring.
		<ol>
			<li>Ask the patient to lie down on his back. Ask him to hold his head in midline position and turn it from one side to the other. In this and all subsequent steps, score the patient on a scale of 0 to 3 (see Table 1) based on performance of the task.</li>
			<li>Ask the patient to lie down on his back with the head in midline position. Ask him to raise the head and maintain the position.</li>
			<li>Ask the patient to lie down on his back. Ask him to bring one knee to the chest.</li>
			<li>Ask the patient to lie down on his back with one leg flexed both at the hip and knee at approximately 90&#176;. Ask him to place the lower leg parallel to the mat with the foot in plantar flexion. Ask him to perform a maximal dorsiflexion of the foot.</li>
			<li>Ask the patient to lie down on his back. Ask him to place one upper limb beside his body and to bring the hand to the opposite shoulder.</li>
			<li>Ask the patient to lie down on his back with the lower limbs half-flexed and the feet resting on the mat slightly apart. Ask him to maintain this position and raise the pelvis.</li>
			<li>Ask the patient to lie down on his back. Ask him to turn over onto the stomach and free both upper limbs.</li>
			<li>Ask the patient to lie down on his back. Ask him to sit up.</li>
			<li>Ask the patient to sit on the mat. Ask him to maintain the seated position and keep the hands in contact in front of the trunk.</li>
			<li>Ask the patient to sit on the mat and place a tennis ball in front of him. Ask him to touch the ball and sit back again. 
			Note: The tennis ball should be at a distance so that the patient must lean his trunk forward about 30&#176; away from the starting position in order to touch it.</li>
			<li>Ask the patient to sit on the mat with the lower limbs in front of him. Ask him to stand up.</li>
			<li>Ask the patient to stand in front of the chair. Ask him to sit down on the chair.</li>
			<li>Ask the patient to sit on the chair. Ask him to maintain the seated position as straight as possible.</li>
			<li>Ask the patient to sit on the chair with the head in complete flexion. Ask him to raise the head and maintain this position.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms (except for the elbows) on the table. Ask him to place both hands on top of the head.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms on the table. Place a pencil on the table and ask him to touch the pencil. 
			Note: The pencil should be placed at a distance equal to the length of the patient&#39;s upper limb.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms on the table. Ask him to pick up the coins next to his hand and to hold them in the same hand. 
			Note: All coins should be placed next to the patient&#39;s hand and picked up successively with one hand.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms on the table. Place a CD glued to a piece of cardboard on the table. Ask him to place one finger in the center of the CD and to trace around the edge of the disc with the finger.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms on the table. Hold a pencil and a paper on the table. Ask him to pick up the pencil and draw inside the frame.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms on the table. Put a sheet of paper in his hands and ask him to tear the paper at least 4 cm.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms on the table. Place a tennis ball next to his hand. Ask him to pick up the ball, raise it, and turn the hand.</li>
			<li>Ask the patient to sit on the chair in front of a table with the forearms on the table. Place a diagram with pictures on the table. Ask him to place the finger at the center of the diagram on the word &#8220;start&#8221;, then place the finger on the drawings.</li>
			<li>Ask the patient to sit on the chair with the arms next to his body and the table at a distance equivalent to the length of his forearm. Ask him to place both hands on the table.</li>
			<li>Ask the patient to sit on the chair with both feet on the ground. Ask him to stand up.</li>
			<li>Ask the patient to stand with the upper limbs resting on a piece of equipment for support. Ask him to release the support and stand straight.</li>
			<li>Ask the patient to stand with the upper limbs resting on a piece of equipment for support. Ask him to release the support and raise one foot.</li>
			<li>Ask the patient to stand without support. Ask him to touch the floor with one hand and stand up again.</li>
			<li>Ask the patient to stand and walk 10 steps on his heels.</li>
			<li>Ask the patient to stand without support. Draw a straight line (about 6 m long and 2 cm wide) on the floor, and ask him to walk on the line.</li>
			<li>Ask the patient to stand without support. Ask him to run.</li>
			<li>Ask the patient to stand on one foot without support with the other foot off the ground. Ask him to hop in place.</li>
			<li>Ask the patient to stand without support. Ask him to squat and stand up again.</li>
		</ol>
		</li>
		<li>Calculate the scores.
		<ol>
			<li>Add the scores of all 32 items, divide the sum by 96, and multiply it by 100 to calculate the final score.</li>
			<li>To calculate scores of the subdomains, add the score of all items in that domain and divide it by the maximum score for the domain, then multiply it by 100. 
			Note: All scores must be calculated as percentages.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>6-minute walk test (6MWT) 
	<ol>
		<li>Task performance
		<ol>
			<li>Let the patient rest for 10 min prior to testing. Demonstrate the walking process.</li>
			<li>Ask the patient to stand at the starting line on the right side of the 0 cone. Give the instructions, &#8220;Ready, set, go&#8221;.</li>
			<li>When saying &#8220;go&#8221;, let the patient start walking around the cones without crossing the middle and, if possible, without slowing down or stopping.</li>
			<li>At 6 min, stop the timer and let the patient stop walking. Count down the final seconds of the test and mark the point at which the patient stopped.</li>
		</ol>
		</li>
		<li>Calculation of the scores
		<ol>
			<li>Record each timepoint at which the patient passes a cone.</li>
			<li>Calculate the total distance by adding a and b, where a is defined as the distance of the final lap (between the last cone rounded until the finishing point at 6 min), and b is defined as the distance in meters prior to the last cone (distance at the time of the last cone rounded).</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Timed 10-meter walk/run test (10MWT) 
	<ol>
		<li>Task performance
		<ol>
			<li>Ask the patient to stand at the starting line. Stand at the 12-meter mark and give the instructions, &#8220;Ready, set, go&#8221;.</li>
			<li>When saying &#8220;go&#8221;, let the patient start walking/running.</li>
			<li>Measure the time and observe the quality of the walk/run. Stop the timer when the second foot of the patient passes the finish line at 10 m.</li>
			<li>Repeat the test three times, and use the fastest performance to calculate the score.</li>
		</ol>
		</li>
		<li>Calculation of the scores
		<ol>
			<li>Score the patient on a 6-point scale (1-6) based on the quality of the walk/run during the fastest trial. Score 1 if he is not able to walk by himself, and score 2 if he is not able to walk by himself but is able to walk when supported by a knee-ankle-foot orthosis or another person.</li>
			<li>Score 3 if he is not able to increase the walking speed and his gait remains highly adapted and lordotic. Score 4 if he is able to increase the walking speed but not able to run while the gait remains moderately adapted.</li>
			<li>Score 5 if he is almost running but cannot raise his feet from the ground. Score 6 if he is able to run and raise both feet from the ground.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Supine-up time
	<ol>
		<li>Task performance
		<ol>
			<li>Ask the patient to lie down on the examination table in supine position. 
			Note: In the case where a mat is necessary, be sure that it is fixed and not slippery.</li>
			<li>Give the instructions, &#8220;Ready, set, go&#8221;. When saying &#8220;go&#8221;, let the patient start standing up as fast as he can.</li>
			<li>Measure the time and observe the quality of the task. Stop the timer when the patient has assumed an upright position with arms by his side. Provide a chair after the patient has attempted to stand from the floor for 30 seconds.</li>
			<li>Repeat the test three times, and use the fastest performance to calculate the score.</li>
		</ol>
		</li>
		<li>Calculation of the scores
		<ol>
			<li>Score the patient on a 6-point scale (1-6). Score 1 if he is not able to stand up from supine position. Score 2 if he is able to stand up from supine position when using a furniture for support.</li>
			<li>Score 3 if he turns over in supine position and needs both hands &#8220;climbing up&#8221; on the legs to reach the standing position. Score 4 if he turns over in supine position and needs one hand on the leg to reach the standing position.</li>
			<li>Score 5 if he turns to the side and uses one or both hands on the ground but not on the leg to reach the standing position. Score 6 if he is able to stand up without turning over or using the hands on the legs.</li>
		</ol>
		</li>
	</ol>
	</li>
</ol>
2. Quantitative Muscle MRI
<ol>
	<li>Perform axial MRI of the thighs including all muscles (flexors, extensor, and adductors) on a 3 Tesla scanner using a 36-channel peripheral angio and spine coil. Perform localizers and slice positioning as previously described11,17.</li>
	<li>Use a three-dimensional (3D) gradient echo sequence with two different echo times for in-phase and opposed-phase imaging [30 slices, repetition time (TR) = 20 ms, echo time 1 (TE1) = 2.45 ms, echo time (TE2) = 3.68 ms, flip angle = 15, acquisition time = 2 min 49 s] and a multi-contrast spin echo with 14 echo times to quantify the transverse relaxation times. Use a field of view of 400 x 400 mm and 384 x 384 matrix to achieve 1 mm in-plane resolution and 3 mm slice thickness.</li>
	<li>Manually draw regions of interest (ROI) on the MR images containing the whole muscle area of flexors, extensors, and adductors of each leg.</li>
	<li>Use the two-point Dixon method and generate relative fat content maps using the pixelwise fat fraction, given f/(f + w), where f = fat images, w = water images17.</li>
	<li>Calculate the T2-relaxation time and mean fat fraction for each muscle group.</li>
</ol>

<ol><li>Ryder, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+burden%2C+epidemiology%2C+costs+and+treatment+for+Duchenne+muscular+dystrophy%3A+an+evidence+review%5BTitle%5D)+AND+%22Orphanet+Journal+of+Rare+Diseases%22%5BJournal%5D)">The burden, epidemiology, costs and treatment for Duchenne muscular dystrophy: an evidence review.</a> Orphanet Journal of Rare Diseases. 12 (1), 79(2017).</li>
<li>Mazzone, E., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Star+Ambulatory+Assessment%2C+6-minute+walk+test+and+timed+items+in+ambulant+boys+with+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Neuromuscular+Disorders%22%5BJournal%5D)">Star Ambulatory Assessment, 6-minute walk test and timed items in ambulant boys with Duchenne muscular dystrophy.</a> Neuromuscular Disorders. 20 (11), 712-716 (2010).</li>
<li>Buckon, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Can+Quantitative+Muscle+Strength+and+Functional+Motor+Ability+Differentiate+the+Influence+of+Age+and+Corticosteroids+in+Ambulatory+Boys+with+Duchenne+Muscular+Dystrophy%3F%5BTitle%5D)+AND+%22PLoS+Currents%22%5BJournal%5D)">Can Quantitative Muscle Strength and Functional Motor Ability Differentiate the Influence of Age and Corticosteroids in Ambulatory Boys with Duchenne Muscular Dystrophy?</a> PLoS Currents. 8, (2016).</li>
<li>Bérard, C., Payan, C., Hodgkinson, I., Fermanian, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((MFM+Collaborative+Study+Group.+A+motor+function+measure+for+neuromuscular+diseases.+Construction+and+validation+study.+Neuromuscular+disorders%5BTitle%5D)+AND+%22NMD%22%5BJournal%5D)">MFM Collaborative Study Group. A motor function measure for neuromuscular diseases. Construction and validation study. Neuromuscular disorders.</a> NMD. 15 (7), 463-470 (2005).</li>
<li>Bérard, C., Vuillerot, C., Girardot, F., Payan, C. MFM Study Group. MFM User's Manual and Score Sheet. 3rd edition erratum revised and corrected November 2017. , Available from:
 <a target="_blank" href="http://www.motor-function-measure.org/user-s-manual.aspx">http://www.motor-function-measure.org/user-s-manual.aspx</a> (2017).</li>
<li>Vuillerot, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Monitoring+changes+and+predicting+loss+of+ambulation+in+Duchenne+muscular+dystrophy+with+the+Motor+Function+Measure%5BTitle%5D)+AND+%22Developmental+Medicine+%26+Child+Neurology%22%5BJournal%5D)">Monitoring changes and predicting loss of ambulation in Duchenne muscular dystrophy with the Motor Function Measure.</a> Developmental Medicine & Child Neurology. 52 (1), 60-65 (2010).</li>
<li>Vuillerot, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Responsiveness+of+the+Motor+Function+Measure+in+Neuromuscular+Diseases%5BTitle%5D)+AND+%22Archives+of+Physical+Medicine+and+Rehabilitation%22%5BJournal%5D)">Responsiveness of the Motor Function Measure in Neuromuscular Diseases.</a> Archives of Physical Medicine and Rehabilitation. 93 (12), 2251-2256 (2012).</li>
<li>McDonald, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+6-minute+walk+test+and+other+clinical+endpoints+in+duchenne+muscular+dystrophy%3A+Reliability%2C+concurrent+validity%2C+and+minimal+clinically+important+differences+from+a+multicenter+study%3A+Reliability%5BTitle%5D)+AND+%22Validity+and+MCID+of+6MWT+and+other+Endpoints+in+DMD.+Muscle+%26+Nerve%22%5BJournal%5D)">The 6-minute walk test and other clinical endpoints in duchenne muscular dystrophy: Reliability, concurrent validity, and minimal clinically important differences from a multicenter study: Reliability.</a> Validity and MCID of 6MWT and other Endpoints in DMD. Muscle & Nerve. 48 (3), 357-368 (2013).</li>
<li>McDonald, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+6-minute+walk+test+as+a+new+outcome+measure+in+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Muscle+%26+Nerve%22%5BJournal%5D)">The 6-minute walk test as a new outcome measure in Duchenne muscular dystrophy.</a> Muscle & Nerve. 41 (4), 500-510 (2010).</li>
<li>ATS Committee on Proficiency Standards for Clinical Pulmonary Function Laboratories. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((ATS+Statement%3A+Guidelines+for+the+Six-Minute+Walk+Test%5BTitle%5D)+AND+%22American+Journal+of+Respiratory+and+Critical+Care+Medicine%22%5BJournal%5D)">ATS Statement: Guidelines for the Six-Minute Walk Test.</a> American Journal of Respiratory and Critical Care Medicine. 166 (1), 111-117 (2002).</li>
<li>Mcdonald, C. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((THE+6-minute+walk+test+and+other+endpoints+in+Duchenne+muscular+dystrophy%3A+Longitudinal+natural+history+observations+over+48+weeks+from+a+multicenter+study%3A+6MWT+and+Endpoints+in+DMD%5BTitle%5D)+AND+%22Muscle+%26+Nerve%22%5BJournal%5D)">THE 6-minute walk test and other endpoints in Duchenne muscular dystrophy: Longitudinal natural history observations over 48 weeks from a multicenter study: 6MWT and Endpoints in DMD.</a> Muscle & Nerve. 48 (3), 343-356 (2013).</li>
<li>Deconinck, N., Dan, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Pathophysiology+of+Duchenne+Muscular+Dystrophy%3A+Current+Hypotheses%5BTitle%5D)+AND+%22Pediatric+Neurology%22%5BJournal%5D)">Pathophysiology of Duchenne Muscular Dystrophy: Current Hypotheses.</a> Pediatric Neurology. 36 (1), 1-7 (2007).</li>
<li>Fischmann, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+MRI+and+loss+of+free+ambulation+in+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Journal+of+Neurology%22%5BJournal%5D)">Quantitative MRI and loss of free ambulation in Duchenne muscular dystrophy.</a> Journal of Neurology. 260 (4), 969-974 (2013).</li>
<li>Barnard, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Skeletal+muscle+magnetic+resonance+biomarkers+correlate+with+function+and+sentinel+events+in+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22PLOS+ONE%22%5BJournal%5D)">Skeletal muscle magnetic resonance biomarkers correlate with function and sentinel events in Duchenne muscular dystrophy.</a> PLOS ONE. 13 (3), e0194283(2018).</li>
<li>Willcocks, R. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Multicenter+prospective+longitudinal+study+of+magnetic+resonance+biomarkers+in+a+large+duchenne+muscular+dystrophy+cohort%3A+Longitudinal+Muscle+MR+in+DMD%5BTitle%5D)+AND+%22Annals+of+Neurology%22%5BJournal%5D)">Multicenter prospective longitudinal study of magnetic resonance biomarkers in a large duchenne muscular dystrophy cohort: Longitudinal Muscle MR in DMD.</a> Annals of Neurology. 79 (4), 535-547 (2016).</li>
<li>Godi, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Longitudinal+MRI+quantification+of+muscle+degeneration+in+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Annals+of+Clinical+and+Translational+Neurology%22%5BJournal%5D)">Longitudinal MRI quantification of muscle degeneration in Duchenne muscular dystrophy.</a> Annals of Clinical and Translational Neurology. 3 (8), 607-622 (2016).</li>
<li>Schmidt, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Timed+function+tests%2C+motor+function+measure%2C+and+quantitative+thigh+muscle+MRI+in+ambulant+children+with+Duchenne+muscular+dystrophy%3A+A+cross-sectional+analysis%5BTitle%5D)+AND+%22Neuromuscular+Disorders%22%5BJournal%5D)">Timed function tests, motor function measure, and quantitative thigh muscle MRI in ambulant children with Duchenne muscular dystrophy: A cross-sectional analysis.</a> Neuromuscular Disorders. 28 (1), 16-23 (2018).</li>
<li>Fischer, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((The+6-minute+walk+test%2C+motor+function+measure+and+quantitative+thigh+muscle+MRI+in+Becker+muscular+dystrophy%3A+A+cross-sectional+study%5BTitle%5D)+AND+%22Neuromuscular+Disorders%22%5BJournal%5D)">The 6-minute walk test, motor function measure and quantitative thigh muscle MRI in Becker muscular dystrophy: A cross-sectional study.</a> Neuromuscular Disorders. 26 (7), 414-422 (2016).</li>
<li>Govoni, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Ongoing+therapeutic+trials+and+outcome+measures+for+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Cellular+and+Molecular+Life+Sciences%22%5BJournal%5D)">Ongoing therapeutic trials and outcome measures for Duchenne muscular dystrophy.</a> Cellular and Molecular Life Sciences. 70 (23), 4585-4602 (2013).</li>
<li>Seferian, A. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Upper+Limb+Strength+and+Function+Changes+during+a+One-Year+Follow-Up+in+Non-Ambulant+Patients+with+Duchenne+Muscular+Dystrophy%3A+An+Observational+Multicenter+Trial%5BTitle%5D)+AND+%22PLOS+ONE%22%5BJournal%5D)">Upper Limb Strength and Function Changes during a One-Year Follow-Up in Non-Ambulant Patients with Duchenne Muscular Dystrophy: An Observational Multicenter Trial.</a> PLOS ONE. 10 (2), e0113999(2015).</li>
<li>Pane, M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Upper+limb+function+in+Duchenne+muscular+dystrophy%3A+24+month+longitudinal+data%5BTitle%5D)+AND+%22PLOS+ONE%22%5BJournal%5D)">Upper limb function in Duchenne muscular dystrophy: 24 month longitudinal data.</a> PLOS ONE. 13 (6), e0199223(2018).</li>
<li>Wokke, B. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+MRI+and+strength+measurements+in+the+assessment+of+muscle+quality+in+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Neuromuscular+Disorders%22%5BJournal%5D)">Quantitative MRI and strength measurements in the assessment of muscle quality in Duchenne muscular dystrophy.</a> Neuromuscular Disorders. 24 (5), 409-416 (2014).</li>
<li>Arpan, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Examination+of+effects+of+corticosteroids+on+skeletal+muscles+of+boys+with+DMD+using+MRI+and+MRS%5BTitle%5D)+AND+%22Neurology%22%5BJournal%5D)">Examination of effects of corticosteroids on skeletal muscles of boys with DMD using MRI and MRS.</a> Neurology. 83 (11), 974-980 (2014).</li>
<li>Hooijmans, M. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Non-uniform+muscle+fat+replacement+along+the+proximodistal+axis+in+Duchenne+muscular+dystrophy%5BTitle%5D)+AND+%22Neuromuscular+Disorders%22%5BJournal%5D)">Non-uniform muscle fat replacement along the proximodistal axis in Duchenne muscular dystrophy.</a> Neuromuscular Disorders. 27 (5), 458-464 (2017).</li>
<li>Carlier, P. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Global+T2+versus+water+T2+in+NMR+imaging+of+fatty+infiltrated+muscles%3A+Different+methodology%2C+different+information+and+different+implications%5BTitle%5D)+AND+%22Neuromuscular+Disorders%22%5BJournal%5D)">Global T2 versus water T2 in NMR imaging of fatty infiltrated muscles: Different methodology, different information and different implications.</a> Neuromuscular Disorders. 24 (5), 390-392 (2014).</li>
<li>Morrow, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((MRI+biomarker+assessment+of+neuromuscular+disease+progression%3A+a+prospective+observational+cohort+study%5BTitle%5D)+AND+%22The+Lancet+Neurology%22%5BJournal%5D)">MRI biomarker assessment of neuromuscular disease progression: a prospective observational cohort study.</a> The Lancet Neurology. 15 (1), 65-77 (2016).</li>
<li>Hollingsworth, K. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/pubmed?term=((Quantitative+MRI+in+muscular+dystrophy%3A+An+indispensable+trial+endpoint%3F%5BTitle%5D)+AND+%22Neurology%22%5BJournal%5D)">Quantitative MRI in muscular dystrophy: An indispensable trial endpoint?</a> Neurology. 83 (11), 956-957 (2014).</li>
</ol>

The authors have nothing to disclose.

Several promising outcome measures have been used in clinical trials in patients with Duchenne muscular dystrophy (DMD). The MFM is a validated and reproducible functional test that involves a detailed examination of crucial motor functions in 32 steps4, while the 6MWT can provide useful information about the patient&#39;s endurance.
All currently validated tests have limitations due to inter- and intra-rater variabilities and all require cooperation of the patient and ...

Outcome measures that reliably reflect treatment response are an essential requirement of successful clinical trials. Due to the rapid development of new therapeutic strategies, stronger effort has been made to define reproducible as well as sensitive methods that monitor clinical outcomes.
Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder and the most common type of muscular dystrophy in children. It is characterized by severe involvement of predominantly the skeletal and heart muscle and a progressive disease course, with loss of ambulation around 8-12 years old and premature death mainly before 30 years old1. Validated tests such as motor function measure and timed function tests are widely accepted as clinical tools for monitoring disease progression, as they assess many aspects of daily life functions. Furthermore, in ambulatory cases, they seem to be more sensitive than quantitative muscle strength measures, which cannot be appropriately performed in weak and non-cooperative patients2,3.
The motor function measure (MFM) assesses functions of the neck, trunk, arm, and leg muscles and abilities such as standing, transferring, and walking. It can even be performed in patients who have lost ambulation, as it reflects three dimensions of motor performance4. The MFM (validated for patients aged 6-60 years with DMD) was evaluated based on the MFM User&#39;s Manual5. It includes 32 items and is divided into three subdomains: D1 (assessment of standing and transfer), D2 (assessment of axial and proximal motor function), and D3 (assessment of distal motor function). All items are scored on a 4-point scale (0-3). The test is validated in neuromuscular disorders and can sufficiently monitor changes in muscle function and predict loss of ambulation. Moreover, it agrees with clinical changes perceived by the treating physicians and patients with DMD6,7. Timed function tests are also commonly used as outcome measures, though they are mainly performed in ambulatory patients. Among these, the 6-minute walk test (6MWT) has received special attention since it shows the highest test-retest reliability, predicts clinical decline and loss of ambulation, and correlates more accurately with muscle function measures compared to quantitative muscle strength measures8,9. The test measures the maximal walking distance of a patient in 6 minutes10. It is guided by two trained professionals, a &#34;follower&#34; who walks 1-2 meters behind the patient, and an &#34;evaluator&#34; who records the time. Other timed function tests have lower test-retest reliability and do not reflect endurance, an important marker of ambulatory function8,9,10,11. These tests include the timed 10-meter run/walk test (10MWT), which measures the best performance of walking/running for a 10-meter distance, and the supine-up time, which measures the ability to stand up from supine position2. The use of the motor function measure and timed function tests as primary and secondary endpoints in clinical trials is justified; however, a major limitation is that none are independent of patient collaboration and the skills of the evaluator.
Quantitative MRI (QMRI) is an objective method to visualize well-described morphological abnormalities of the musculature including edema, muscle degeneration, and increased content of adipose and connective tissue12. The use of MRI as a diagnostic tool in neuromuscular disorders has already been established, but its role in monitoring disease progression and treatment response is still limited to clinical trials. T2-relaxation time is known to be increased in muscle dystrophies due to muscle damage, edema, fatty replacement, and inflammation, and further information about muscle fat content can be extracted through calculation of the mean fat fraction (FF). QMRI has been shown to be a promising biomarker, as measurements have correlated with clinical outcome and disease progression, while a mean fat fraction of 50% predicted loss of ambulation13,14. Moreover, QMRI has been able to detect subclinical changes in patients with stable or even improved outcome measures15,16. QMRI data of extensors muscles has also shown to be meaningful regarding its correlation with clinical outcomes17. QMRI is a non-invasive and sensitive method; nevertheless, its cost and the possibility of reduced compliance in younger children may limit its use.
The reliability of functional tests and QMRI has been previously shown in Becker&#39;s muscular dystrophy18. The aim of this cross-sectional study was to highlight sensitive clinical and radiological outcome measures in ambulant children with DMD, as the need for standardized and disease-specific assessments is increasing in the era of clinical trials in neuromuscular disorders.

<table><tbody><tr><td>Physiotherapy mat</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>should not be slippery; alternatively use a wide examination table</td></tr><tr><td>Cushions</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td></tr><tr><td>Table&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>with adjustable height; it should allow the patient to rest forearms while seated and elbows flexed at 90&amp;deg;</td></tr><tr><td>Chair</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>with adjustable height if possible; it should allow the patient to touch the floor with the feet while seated with the hips and knees flexed at 90&amp;deg;</td></tr><tr><td>Stopwatch</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td></tr><tr><td>CD or CD-ROM glued onto a piece of cardboard</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td></tr><tr><td>10 coins&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>dimensions: 20 mm wide and 2 mm thick (10 euro cents or equivalent)</td></tr><tr><td>Lead pencil</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td></tr><tr><td>Tennis ball</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td></tr><tr><td>Sheets of A4 paper or equivalent&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>weight: 70-80g</td></tr><tr><td>Clipboard</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td></tr><tr><td>Two small traffic cones&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td></tr><tr><td>Tape&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>for marking arrows and stop&amp;nbsp;&amp;nbsp;</td></tr><tr><td>Line traced on the floor</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>2 centimeters wide and 6 meters long</td></tr><tr><td>Corridor</td><td>&amp;nbsp;-&amp;nbsp;</td><td>&amp;nbsp;-&amp;nbsp;</td><td>indoor, straight, up to 30 meters long&amp;nbsp;</td></tr></tbody></table>

measurements of motor function and other clinical outcome parameters in ambulant children with duchenne muscular dystrophy

Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy

While the number of new treatment options tested in patients with Duchenne muscular dystrophy (DMD) is increasing, there is still no defining of the most reliable assessments regarding therapeutic efficacy. We present clinical and radiological outcome measures used in ambulatory patients participating in our trial &#34;Treatment with L-citrulline and metformin in Duchenne muscular dystrophy&#34;. The motor function measure is a validated test in patients with neuromuscular disorders that consists of 32 items and assesses all three dimensions of motor performance including standing and transfer (D1 subscore), axial and proximal motor function (D2 subscore), and distal motor function (D3 subscore). The test shows high intra- and inter-rater variability but only when strictly following guidelines of the materials, examination steps, and calculation of scores. The 6-minute walk test, timed 10-meter walk/run test, and supine-up time are commonly used timed functional tests that also sufficiently monitor changes in muscle function; however, they strongly depend on patient collaboration. Quantitative MRI is an objective and sensitive biomarker to detect subclinical changes, though the examination costs may be a reason for its limited use. In this study, a high correlation between all clinical assessments and quantitative MRI scans was found. The combinational use of these methods provides a better understanding about disease progression; however, longitudinal studies are needed to validate their reliability.

Baseline data of 47 ambulatory male patients (aged 6.5 to 10.8 years) with DMD were analyzed. All patients participated previously in the "Treatment with L-citrulline and metformin in Duchenne muscular dystrophy" study. Patients were enrolled from the University Children's Hospital Basel and from the patient registries of Switzerland, Germany, and Austria. Except for one patient, who refused to take part in the scanning, MRI of all thigh muscles was performed13<sup...

The aim of this study is to present the most reliable clinical outcome measures and their correlations with quantitative muscle MRI in ambulant patients with Duchenne muscular dystrophy.

While the number of new treatment options tested in patients with Duchenne muscular dystrophy (DMD) is increasing, there is still no defining of the most ...

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Research

JoVE Journal

Medicine

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Learn motor function assessment for Duchenne muscular dystrophy using standardized clinical outcome measures. This method evaluates ambulant children through scored tasks and correlates results with quantitative muscle MRI findings.

Measurements of Motor Function and Other Clinical Outcome Parameters in Ambulant Children with Duchenne Muscular Dystrophy

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