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JoVE Journal Neuroscience
Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
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Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

Article DOI: 10.3791/58788-v • 07:27 min • January 31st, 2019
January 31st, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, *1,2,3, *1,2,3
1Institut National de la Santé et de la Recherche Médicale (INSERM), UMR-S 1270 Paris, France, 2Sorbonne Université, Paris, France, 3Institut du Fer à Moulin, Paris, France
* These authors contributed equally

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Summary
Automatic Translation

Microglia, the resident immune cells of the brain, respond quickly with morphological changes to modifications of their environment. This protocol describes how to use two-photon microscopy to study the attraction of microglial processes toward serotonin or ATP in acute brain slices of mice.

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Two-photon Imaging Microglial Processes ATP Serotonin Acute Brain Slices Directional Motility Mice Brain Slices 3D Print Interface Perfusion Chambers Fanny Etienne Vincenzo Mastriolia Choline Artificial Cerebrospinal Fluid ACSF 3D Printed Interface Slice Holder Crystallizing Dish Bar Magnet Carbogen Food Box Humidified Interface Chamber Brain Dissection Coronal Slicing
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