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In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration

Article DOI: 10.3791/58838-v • 09:24 min • February 23rd, 2019
February 23rd, 2019 •
1,4, 1, 1, 1,3, 1,2,4
1Department of Biological Sciences, Northern Arizona University, 2Department of Mechanical Engineering, Northern Arizona University, 3Department of Chemistry and Biochemistry, Northern Arizona University, 4Center for Bioengineering Innovation, Northern Arizona University

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This study focuses on an in vitro model of wound healing (scratch assay) as a mechanism for determining how environmental contaminants such as arsenic influence cellular migration. The results demonstrate that this in vitro assay provides rapid and early indications of changes to cellular migration prior to in vivo experimentation.

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In Vitro Scratch Assay Arsenic Skin Cell Migration Wound Healing Compounds Targeted Treatment Strategies Clinical Medicine Cost-effective High-throughput In Vivo Testing Migration Assay Cell Type Standard Operating Procedures Training Technique Practice Biosafety II Cabinet 70% Isopropyl Aqueous Solution Sanitation Wipe Cryopreserved Cell Line
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