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Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets
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Neuroscience
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JoVE Journal Neuroscience
Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

Autoradiography as a Simple and Powerful Method for Visualization and Characterization of Pharmacological Targets

DOI:
10.3791/58879-v

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10:16 min

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March 12, 2019

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, , ,
1Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences,University of Copenhagen, 2Neurobiology Research Unit and CIMBI,Copenhagen University Hospital, 3Department of Clinical Physiology, Nuclear Medicine & PET,Copenhagen University Hospital

Chapters

  • 00:04Title
  • 00:46Tissue Preparation by Cryosectioning
  • 02:13In vitro Autoradiography
  • 04:44Exposure to Phosphor Imaging Plates and Scanning
  • 06:04Densitometric Analysis of Digital Image
  • 07:34Results: Visualization and Characterization of Pharmacological Targets Using Radiography
  • 09:34Conclusion

Summary

Automatic Translation

Automatic Translation

The method of autoradiography is routinely used to study binding of radioligands to tissue sections for determination of qualitative or quantitative pharmacology.

Tags

Keywords: AutoradiographyProtein BindingPharmacological TargetsCryostat SectioningRadioligand LabelingNon-specific BindingSpatial ResolutionDigital ImagingPharmacological Analysis

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