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JoVE Journal
Biochemistry

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A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity
 

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Article doi: 10.3791/58892
January 9th, 2019

Summary January 9th, 2019

We present a fluorogenic peptide cleavage assay that allows a rapid screening of the proteolytic activity of proteases on peptides representing the cleavage site of viral fusion peptides. This method can also be used on any other amino acid motif within a protein sequence to test for the protease activity.

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