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A New Portable In Vitro Exposure Cassette for Aerosol Sampling
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A New Portable In Vitro Exposure Cassette for Aerosol Sampling

A New Portable In Vitro Exposure Cassette for Aerosol Sampling

DOI:
10.3791/58916-v

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07:01 min

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February 22, 2019

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, ,
1Department of Chemical and Life Science Engineering,Virginia Commonwealth University

Chapters

  • 00:04Title
  • 00:39Generation of Dry Aerosol
  • 02:21Portable In Vitro Exposure Cassette (PIVEC) Assembly
  • 03:45Aerosol Exposure of Cells
  • 04:52Results: Deposition Efficiency and Expedited Post-exposure Analysis
  • 06:00Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.

Tags

Keywords: In Vitro ExposureAerosol SamplingPIVECPortable Exposure SystemAir Quality MonitoringLung Cell CultureDry Dispersal SystemCopper NanoparticlesHEPA FilterVacuum GeneratorAir-liquid InterfaceCell Culture Insert

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