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Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System

Live Cell Analysis of Shear Stress on Pseudomonas aeruginosa Using an Automated Higher-Throughput Microfluidic System

DOI:
10.3791/58926-v

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09:12 min

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January 16, 2019

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, , , , , , , ,
1Department of Chemistry,Doane University, 2Department of Biology,Doane University, 3Department of Pathology and Microbiology,University of Nebraska Medical Center, 4Department of Physics and Engineering,Doane University

Chapters

  • 00:04Title
  • 00:47Equipment Startup
  • 01:27Microfluidic Plate Priming and Seeding
  • 03:39Software and Timed Biofilm Growth Experiment Setup
  • 07:02Results: Representative Percent Threshold Area, Total Biofilm Accumulation, Roughness Coefficient, and Textural Entropy
  • 08:12Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe the use of a higher-throughput microfluidic bioreactor coupled with a fluorescent microscope for the analysis of shear stress effects on Pseudomonas aeruginosa biofilms expressing green fluorescent proteins, including instrument set up, the determination of biofilm coverage, growth rate, and morphological properties.

Tags

Keywords: Live Cell AnalysisShear StressPseudomonas AeruginosaAutomated Higher-throughput Microfluidic SystemBiofilm DevelopmentEnvironmental ConditionsBiofilm GrowthBehavioral CharacteristicsMulti-channeled Microfluidic PlatesStatistically Significant ResultsBiofilm StructureAntibiotic TreatmentsBioremediationProtocolFluorescence ModuleImaging System ControllerCCD CameraImaging StationMinimal MediumLuria-Bertani BrothShear Stress

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