Journal
/
Immunology and Infection
/
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

DOI:
10.3791/58929-v

•

10:26 min

•

January 20, 2019

•

, , , ,
1IGH, CNRS,Univ Montpellier, 2Laboratory for Monitoring Innovative Therapies, Department of Biological Hematology,CHU Montpellier, 3UFR de Médecine,Univ Montpellier

Chapters

  • 00:04Title
  • 00:52B Cell Amplification and Differentiation
  • 03:44Plasmablastic Cell Generation
  • 04:36Early Plasma Cell Generation
  • 05:21Long-lived Plasma Cell Generation
  • 06:29Molecular Atlas of B to PC Differentiation
  • 07:33Results: Epigenetic Factor Expression in Normal Plasma Cell Differentiation
  • 09:01Conclusion

Summary

Automatic Translation

Automatic Translation

Using multi-step culture systems, we report an in vitro B cell to plasma cell differentiation model.

Tags

Keywords: In Vitro DifferentiationHuman Normal Memory B CellsLong-lived Plasma CellsPlasma Cell DifferentiationB2 Plasma Cell DifferentiationTranscriptional ChangesPhenotypeBioinformatic ToolsMemory B Cell PurificationCD40 LigandAnti-polyhistidine Monoclonal Antibody

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58929?article_type=v

Murine Model of CD40-activation of B cells
check_url/58929?article_type=v

Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
check_url/58929?article_type=v

An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells
check_url/58929?article_type=v

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
check_url/58929?article_type=v

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
check_url/58929?article_type=v

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
check_url/58929?article_type=v

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
check_url/58929?article_type=v

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
check_url/58929?article_type=v

The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
check_url/58929?article_type=v

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

Read Article