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Bioengineering
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使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
Please note that all translations are automatically generated.
Click here for the English version.
使用自动微生物反应器系统从中国仓鼠卵巢细胞中纯化和分析单克隆抗体
DOI:
10.3791/58947-v
•
10:50 min
•
May 01, 2019
•
Sai Rashmika Velugula-Yellela
,
David N. Powers
,
Phillip Angart
,
Anneliese Faustino
,
Talia Faison
,
Casey Kohnhorst
,
Erica J. Fratz-Berilla
,
Cyrus D. Agarabi
1
Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II
,
U.S. Food and Drug Administration
Chapters
00:04
Title
00:39
Antibody Purification
01:49
Purified Antibody Concentration
02:33
N-Glycan Labeling and Isolation
05:00
Labeled N-Glycan Liquid Chromatography-Mass Spectrometry (LC-MS) Analysis
06:17
Charge Variant Sample Preparation
07:38
Charge Variant Chip Preparation
08:31
Results: Representative Purified Monoclonal Antibody Automated Microbioreactor System Analyses
09:58
Conclusion
Summary
Automatic Translation
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Automatic Translation
介绍了从自动微生物反应器的收获细胞培养液(HCCF)中分离和后续分析的单克隆抗体的详细方案。还介绍了使用分析来确定关键质量属性 (CQA) 和最大化有限样本量以提取重要信息。
Tags
Monoclonal Antibody
Chinese Hamster Ovary Cells
Automated Microbioreactor System
Purification
Analytics
Critical Quality Attributes
N-glycan Labeling
Liquid Chromatography Mass Spectrometry (LC-MS)
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