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Journal / Genetics / Raising the Mexican Tetra Astyanax mexicanus for…
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Genetics

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JoVE Journal Genetics
Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry
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Raising the Mexican Tetra Astyanax mexicanus for Analysis of Post-larval Phenotypes and Whole-mount Immunohistochemistry

Article DOI: 10.3791/58972-v • 06:42 min • December 28th, 2018
December 28th, 2018 •
1, 1, 1, 1
1Harvard Medical School

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In this protocol, we demonstrate how to breed Astyanax mexicanus adults, raise the larvae, and perform whole-mount immunohistochemistry on post-larval fish to compare the phenotypes of surface and cave morphotypes.

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Mexican Tetra Astyanax Mexicanus Breed Larvae Whole-mount Immunohistochemistry Post-larval Phenotypes River Fish Cave Fish Plastic Mesh Five Gallon Tank Water Heater Nutrient Rich Diet Rotifers Consistent Growth Cost Advantages Biosecurity Advantages Water Quality Advantages Female Fish Male Fish Temperature Control Flashlight Check For Eggs Beaker Water Depth Reduction Fertilization Time Estimation Transfer Pipette Petri Dish Stereo Microscope Opaque Eggs Removal Methylene Blue Addition Heater And Aquarium Bubbler
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