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JoVE Journal
Genetics

This content is Open Access.

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria
 

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria

Article doi: 10.3791/58981
January 25th, 2019 Usage Statistics

Summary January 25th, 2019

Dictyostelium discoideum is a popular model organism to study complex cellular processes such as cell migration, endocytosis, and development. The utility of the organism is dependent on the feasibility of genetic manipulation. Here, we present methods to transfect Dictyostelium discoideum cells that overcome existing limitations of culturing cells in liquid media.

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