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A Mouse Model to Assess Innate Immune Response to Staphylococcus aureus Infection
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Chapters
- 00:04Title
- 00:38Bacterial Preparation and Quantification
- 02:44Excisional Skin Wounding
- 03:50Staphylococcus aureus Inoculation
- 04:44In Vivo Bioluminescent and Fluorescence Imaging
- 06:02Bioluminescent and Fluorescence Image Analysis
- 06:45Results: LysM-EGFPMyD88-/- Mice are More Susceptible to S. aureus Infection Than LysM-EGFP Mice
- 08:30Conclusion
An approach is described for real-time detection of the innate immune response to cutaneous wounding and Staphylococcus aureus infection of mice. By comparing LysM-EGFP mice (which possess fluorescent neutrophils) with a LysM-EGFP crossbred immunodeficient mouse strain, we advance our understanding of infection and the development of approaches to combat infection.
