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Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
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JoVE Journal Environment
Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

DOI:
10.3791/59035-v

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09:38 min

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January 07, 2019

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, , , , , , ,
1Environmental Molecular Sciences Laboratory,Pacific Northwest National Laboratory, 2Department of Soil, Water and Environmental Science,University of Arizona, 3Department of Earth Ocean and Atmospheric Sciences,Florida State University, 4Bruker Daltonics Inc., 5Biological Sciences Division,Pacific Northwest National Laboratory

Chapters

  • 00:04Title
  • 01:11Sequential Extraction of Organic Matter from Soil, Sediments, or Peat
  • 02:42FTICR-MS Analysis
  • 04:55Chemical Derivatization for GC-MS
  • 06:14Liquid State NMR Analysis and LC-MS Lipidomics Analysis
  • 07:22Proteomics Analysis
  • 07:37Results: Number of Enzymes Identified in Minnesota Peat at Three Different Depths
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a single throughput for complementary analytical and omics techniques culminating in a fully-paired characterization of natural organic matter and microbial proteomics in different ecosystems. This approach permits robust comparisons for identifying metabolic pathways and transformations important for describing greenhouse gas production and predicting responses to environmental change.

Tags

Keywords: Natural Organic MatterMulti-omicsMass SpectrometryNMR SpectroscopyMetabolomicsProteomicsLipidomicsSequential ExtractionMPLEx ExtractionBioavailable CompoundsPolar CompoundsNon-polar CompoundsSample Preparation

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