Sequential Extraction
Sequential Extraction - Related Videos
07:41
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
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Cited by 20 •
2017
Sequential salt extraction of chromatin bound proteins is a useful tool for determining the binding properties of large protein complexes. This method can be employed to evaluate the role of individual subunits or domains in the overall affinity of a protein complex to bulk chromatin.
09:27
Sequential Extraction of Soluble and Insoluble Alpha-Synuclein from Parkinsonian Brains
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Cited by 46 •
2016
Here, we present a protocol for the isolation of increasingly insoluble/aggregated alpha-synuclein (α-syn) from post-mortem human brain tissue. Through the utilization of buffers with increasing detergent strength and high-speed ultracentrifugation techniques, the variable properties of α-syn aggregation in diseased and non-diseased tissue can be examined.
01:21
Sequential Games
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2025
Sequential games involve players making decisions one after another, with each player considering the prior decisions of other players before deciding their own strategy. The order of these decisions is crucial, as each decision influences subsequent ones, affecting the overall outcome. Decision trees are valuable tools in illustrating these games, helping visualize each possible decision and its consequences, allowing players to anticipate and plan strategies effectively. Consider two coffee...
01:24
Sequential Game: Backward Induction
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2025
Backward induction is a technique for solving sequential games. It involves analyzing the game starting from the end and working backwards to the beginning. This method helps players determine their best strategies by anticipating how others will react at each stage of the game, ultimately leading to the Nash equilibrium. Imagine two beverage companies, FreshFizz and CoolBrew, deciding whether to enter a new market. FreshFizz moves first and must choose to enter or stay out. If FreshFizz...
09:52
Electricity-Free, Sequential Nucleic Acid and Protein Isolation
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Cited by 4 •
2012
A tool and chemistries are described to sequentially isolate nucleic acids followed by proteins from a sample without the need for electricity. The tool consists of a sorbent held within a transfer pipette while the isolation chemistries are based on solid-phase extraction principles. The isolated macromolecules can be analyzed by immuno-based and PCR-based assays.
FAQs
The solvent sequence exploits differences in solubility and polarity. A solvent dissolves a subset of compounds more effectively than others, allowing that fraction to be removed while less-compatible material remains. Changing solvent polarity in later steps broadens the range of compounds recovered. This staged selectivity helps distinguish chemical classes that would be mixed in a single complex extract.
At each stage, the residual sample contains compounds not efficiently removed by the preceding solvent. Treating that material again with a different solvent can enrich another chemical class rather than discarding it with the first fraction. In biochemical samples, this supports separate examination of metabolites, lipids, pigments, and other biomolecules.
Compared with a single extraction, Sequential extraction creates several fractions instead of one combined mixture. That division can reduce sample complexity and make compounds easier to examine in later analyses. The benefit depends on choosing solvents whose polarity differences produce useful selectivity; the method does not automatically provide complete separation of every compound in the original sample.
A basic workflow applies one solvent to the sample, removes the resulting extract, and subjects the remaining material to another solvent treatment. The sequence continues with solvents selected for differing polarities and solubility behavior. Each fraction should remain associated with its extraction step, because the treatment order provides information about how compounds were partitioned across the sample.
Fractions from Sequential extraction can be directed to chromatography, where reduced chemical complexity supports further separation or measurement. They may also contribute to structure elucidation, the process of determining chemical identity or arrangement, and to bioactivity screening. Because each fraction represents a different extraction stage, analytical results can be related to the compounds enriched in that fraction.
In biochemistry, the method is useful when a sample contains chemically diverse constituents, such as metabolites, lipids, pigments, and other biomolecules. Fractionation helps investigators study biochemical composition without treating the sample as a single undifferentiated mixture. The resulting fractions can support qualitative analysis, which characterizes constituents, and quantitative analysis, which measures them.