Journal
/
Chemistry
/
Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction
JoVE Journal
Chemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Chemistry
Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

Pretargeted Radioimmunotherapy Based on the Inverse Electron Demand Diels-Alder Reaction

DOI:
10.3791/59041-v

•

09:44 min

•

January 29, 2019

•

, ,
1Department of Chemistry,Hunter College of the City University of New York, 2Ph.D. Program in Chemistry,Graduate Center of the City University of New York, 3Department of Radiology,Memorial Sloan Kettering Cancer Center, 4Department of Radiology,Weill Cornell Medical College

Chapters

  • 00:04Title
  • 00:41The Preparation of huA33-TCO
  • 03:54177Lu Radiolabeling of Tz-PEG7-DOTA
  • 05:10In vivo Studies
  • 07:08Results: Longitudinal Therapy Study of Mice Bearing Subcutaneous SW1222 Tumors
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the synthesis and characterization of a trans-cyclooctene (TCO)-modified antibody and a 177Lu-labeled tetrazine (Tz) radioligand for pretargeted radioimmunotherapy (PRIT). In addition, it details the use of these two constructs for in vivo biodistribution and longitudinal therapy studies in a murine model of colorectal cancer.

Tags

Keywords: Pretargeted RadioimmunotherapyPRITInverse Electron Demand Diels-Alder ReactionColorectal Cancer XenograftAntibody ConjugationTCO-NHSHuA33 AntibodySize Exclusion ChromatographyUV-vis Spectrophotometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59041?article_type=v

Microwave-assisted Intramolecular Dehydrogenative Diels-Alder Reactions for the Synthesis of Functionalized Naphthalenes/Solvatochromic Dyes
check_url/59041?article_type=v

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
check_url/59041?article_type=v

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
check_url/59041?article_type=v

Amide Coupling Reaction for the Synthesis of Bispyridine-based Ligands and Their Complexation to Platinum as Dinuclear Anticancer Agents
check_url/59041?article_type=v

An Inverse Analysis Approach to the Characterization of Chemical Transport in Paints
check_url/59041?article_type=v

The Preparation and Properties of Thermo-reversibly Cross-linked Rubber Via Diels-Alder Chemistry
check_url/59041?article_type=v

A Rapid Synthesis Method for Au, Pd, and Pt Aerogels Via Direct Solution-Based Reduction
check_url/59041?article_type=v

A Salt-Templated Synthesis Method for Porous Platinum-based Macrobeams and Macrotubes
check_url/59041?article_type=v

Quantitative Atomic-Site Analysis of Functional Dopants/Point Defects in Crystalline Materials by Electron-Channeling-Enhanced Microanalysis
check_url/59041?article_type=v

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Read Article