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A High-content Assay for Monitoring AMPA Receptor Trafficking
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Neuroscience
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JoVE Journal Neuroscience
A High-content Assay for Monitoring AMPA Receptor Trafficking

A High-content Assay for Monitoring AMPA Receptor Trafficking

DOI:
10.3791/59048-v

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10:34 min

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January 28, 2019

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, ,
1Neuroscience Institute,Georgia State University

Chapters

  • 00:04Title
  • 01:17Preparation of Primary Hippocampal Neurons and Measuring AMPA Receptor Trafficking in Response to DHPG
  • 02:26Immunolabelling (performed in Laminar Flow Hood)
  • 04:09Labeling Surface Receptors
  • 05:07Labeling the Internalized Receptor Population
  • 06:18Imaging and Analysis
  • 08:07Results: Analysis of AMPA Receptor Trafficking Using the High-content Assay
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

Neuronal excitability can be modulated through a dynamic process of endo- and exocytosis of excitatory ionotropic glutamate receptors. Described here is an accessible, high-content assay for quantifying surface and internal receptor population pools.

Tags

Keywords: AMPA Receptor TraffickingHigh-content AssayPrimary Neuronal CultureSurface And Intracellular Receptor PoolsReceptor Trafficking ProfilesSynaptic LossAlzheimer’s DiseaseTTX TreatmentAnti-GluA1/GluA2 Antibody

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