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原发性人类 CD4+ T 淋巴细胞低线粒体 DNA 污染的 ATAC-seq 检测
JoVE Journal
Genetics
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JoVE Journal Genetics
ATAC-seq Assay with Low Mitochondrial DNA Contamination from Primary Human CD4+ T Lymphocytes
DOI:

08:36 min

March 22, 2019

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Chapters

  • 00:04Title
  • 00:55Activation and Purification of CD4+ T Cells
  • 04:36Assay for Transposase-accessible Chromatin Sequencing (ATAC-seq)
  • 06:33Results: ATAC-seq and Sequencing Result Analysis
  • 07:48Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 以执行检测转座受接触染色质测序 (ATAC-seq) 激活 CD4 + 人类淋巴细胞。该协议已被修改, 以尽量减少污染线粒体 DNA 读取从50% 到3% 通过引入一个新的裂解缓冲液。

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