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Journal / Bioengineering / Küresel hücresel matriks metalloproteinaz ve 3D…
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Bioengineering

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JoVE Journal Bioengineering
Küresel hücresel matriks metalloproteinaz ve 3D Hydrogels metabolik etkinliğini ölçme
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Küresel hücresel matriks metalloproteinaz ve 3D Hydrogels metabolik etkinliğini ölçme

Article DOI: 10.3791/59123-v • 07:39 min • January 22nd, 2019
January 22nd, 2019 •
1,2,3, 1,2
1Department of Biomedical Engineering, The Ohio State University, 2The Ohio State University Comprehensive Cancer Center, Arthur G. James Cancer Hospital and Richard J. Solove Research Institute, 3Biomedical Technology Department, King Saud University

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Burada, bir protokol Kapsüllenen ve bir fluorogenic matriks metalloproteinaz (MMP) functionalized poly(ethylene glycol) (PEG) hydrogels hücrelerde kültür sunulan-parçalanabilir peptid. Hücresel MMP ve metabolik aktivite doğrudan bir standart Mikroplaka Okuyucu kullanarak hidrojel kültürler ölçülür.

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Kapsülleme matriks metalloproteinazların metabolik aktivite melanom floresans Biyomühendislik sayı 143 Hydrogels 3D hücre
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