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1Longhua Hospital,Shanghai University of Traditional Chinese Medicine, 2Spine Institute,Shanghai University of Traditional Chinese Medicine, 3Key Laboratory of Theory and Therapy of Muscles and Bones, Ministry of Education, 4School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine
Chapters
- 00:04Title
- 00:41Gushukang (GSK) Working Solution Preparation and Administration
- 01:18Intragastric Administration
- 02:13GSK-Containing Blood Serum Preparation
- 03:42In Vitro Osteoclastogenesis and Osteoblastogenesis
- 04:30Results: Representative Effects of GSK on Bone Loss and Bone Cell Genesis
- 05:39Conclusion
This article provides a detailed protocol for preparing a working solution of Gushukang granules for animal studies and GSK granule containing serum for in vitro experiments. This protocol can be applied to pharmacological investigations of herbal medicines as well as prescriptions for both in vivo and in vitro experiments.
