In Vivo Experiments
In Vivo Experiments - Related Videos
10:15
Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment
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Cited by 2 •
2013
Study of the animal organ physiology can be achieved by performing an experimental ex vivo perfusion system. The addition of a porcine kidney, as a homeostatic organ to our previously developed ex vivo liver perfusion model can be a principal step to achieve a better physiological environment.
07:03
"Avatar", a Modified Ex vivo Work Loop Experiments Using In vivo Strain and Activation
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Cited by 5 •
2023
This article details the methodology for emulating in vivo muscle force production during ex vivo work loop experiments using an "avatar" muscle from a laboratory rodent to assess the contributions of strain transients and activation to the muscle force response.
10:07
In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
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Cited by 31 •
2013
Experience-dependent molecular changes in neurons are essential for the brain's ability to adapt in response to behavioral challenges. An in vivo two-photon imaging method is described here that allows the tracking of such molecular changes in individual cortical neurons through genetically encoded reporters.
08:09
Validation of Nanobody and Antibody Based In Vivo Tumor Xenograft NIRF-imaging Experiments in Mice Using Ex Vivo Flow Cytometry and Microscopy
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Cited by 24 •
2015
This protocol outlines the steps required to perform ex vivo validation of in vivo near-infrared fluorescence xenograft imaging experiments in mice using fluorophore labelled nanobodies and conventional antibodies.
06:16
Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments
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Cited by 4 •
2019
This article provides a detailed protocol for preparing a working solution of Gushukang granules for animal studies and GSK granule containing serum for in vitro experiments. This protocol can be applied to pharmacological investigations of herbal medicines as well as prescriptions for both in vivo and in vitro experiments.
FAQs
Physiological context can change how an engineered intervention behaves. Interactions among organs, cells, and the immune system may influence observed responses in ways that isolated laboratory findings do not capture. Measuring these integrated effects helps researchers determine whether a design performs under conditions relevant to its intended biological use.
Key outcomes include biocompatibility, safety, performance, and therapeutic efficacy. Together, these measures show whether the organism tolerates a device, material, therapy, or engineered system, whether it functions as intended, and whether it produces the desired biological effect. Evaluating all four supports more informed design refinement and translation decisions.
A response observed at the target site may reflect broader biological interactions rather than the intervention alone. Cells, organs, and the immune system can influence one another throughout the study, affecting measured outcomes. Accounting for these relationships helps researchers interpret findings in physiological context and identify limitations that simpler laboratory systems may not reveal.
Researchers introduce or observe a device, material, therapy, or engineered system in an organism, then monitor its effects using suitable measurements. These may include imaging, sampling, functional measurements, and tissue analysis. The resulting observations are examined together to assess biological responses, system performance, safety, and therapeutic effects.
In vivo evidence becomes especially valuable when researchers need to examine whether laboratory findings remain valid in a realistic biological setting. Results can reveal how an intervention performs amid interactions among tissues, cells, organs, and the immune system. This information supports refinement of the design before clinical translation is considered.
The approach supports evaluation of implants, drug-delivery systems, tissue-engineered constructs, and regenerative medicine strategies. For these applications, researchers can examine compatibility with the organism, functional behavior, tissue responses, and therapeutic effects. Such evidence helps connect engineered designs with their intended biological use and informs decisions about further development.