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JoVE Journal Bioengineering
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
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Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model

Article DOI: 10.3791/59173-v • 09:57 min • April 1st, 2019
April 1st, 2019 •
*1,2, *1,3, 1,2, 4, 5, 3,6, 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
* These authors contributed equally

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Summary
Automatic Translation

Graft stenosis poses a critical obstacle in tissue engineered airway replacement. To investigate cellular mechanisms underlying stenosis, we utilize a murine model of tissue engineered tracheal replacement with seeded bone marrow mononuclear cells (BM-MNC). Here, we detail our protocol, including scaffold manufacturing, BM-MNC isolation, graft seeding, and implantation.

Tags

Seeding Implantation Biosynthetic Tissue-engineered Tracheal Graft Mouse Model Clinical Applications Graft Stenosis Delayed Epithelialization Orthotopic Tracheal Replacement Cellular Mechanisms Regeneration Long-segment Defects Current Therapies Replacement Organ Transgenic Animals Lineage Tracing Properties Host Factors Electrospinning Parameters Scaffold Production Tip-to-collector Distance Humidity Temperature Microsurgical Techniques Anesthesia Ventilation Host Trachea Diameter Polyethylene Terephthalate (PET) Hexafluoroisopropanol
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