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JoVE Journal Bioengineering
Såing og implantering av en biosyntetiske vev-konstruert Tracheal pode i en musemodell
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Såing og implantering av en biosyntetiske vev-konstruert Tracheal pode i en musemodell

Article DOI: 10.3791/59173-v • 09:57 min • April 1st, 2019
April 1st, 2019 •
*1,2, *1,3, 1,2, 4, 5, 3,6, 1,3
1Department of Otolaryngology Head & Neck Surgery, Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine, Research Institute at Nationwide Children's Hospital, 4Center for Perinatal Research, Nationwide Children's Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery, Nationwide Children's Hospital
* These authors contributed equally

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Pode stenose utgjør en viktig hindring i vev utvikling airway erstatning. For å undersøke mobilnettet mekanismene bak stenose, benytter vi en murine modell av vev utvikling tracheal erstatning med seeded benmarg mononukleære celler (BM-MNC). Her, detalj vi våre protokollen, inkludert stillaset produksjon, BM-MNC isolasjon, pode såing og implantasjon.

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Bioteknologi problemet 146 electrospinning syntetisk skafottet medisin tracheal pode celle såing biologisk materiale tracheal sykdommer
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