Journal

Biochemistry

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

In Vivo Calcium Imaging in C. elegans Body Wall Muscles

DOI:

10.3791/59175-v

•

08:03 min

•

October 20, 2019

•

Ashley A. Martin², Simon Alford, Janet E. Richmond

¹Department of Biological Sciences,University of Illinois at Chicago, ²Department of Integrative Biology and Physiology,University of Minnesota, ³Department of Anatomy and Cell Biology,University of Illinois at Chicago

Chapters

00:04Title
01:10Microscope Setup
02:57C. elegans Sample Preparation & Data Acquisition
05:16Results: Baseline Calcium Levels & Evoked Calcium Transients in C. elegans Body Wall Muscles
07:03Conclusion

Summary

Automatic Translation

This method provides a way to couple optogenetics and genetically encoded calcium sensors to image baseline cytosolic calcium levels and changes in evoked calcium transients in the body wall muscles of the model organism C. elegans.

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