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Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
This content is Free Access.
1Department of Integrated Traditional Chinese and Western Medicine,West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute,Arizona State University, 3School of Biological and Health Systems Engineering,Arizona State University, 4Department of Electrical and Computer Engineering,North Dakota State University
Chapters
- 00:04Title
- 01:01Preparation of Nanoparticle Probes (AuNR-IgG)
- 02:17Preparation of Extracellular Vesicle Capture Slides and Exosome Capture
- 05:17Low-Magnification Dark-Field Microscope (LMDFM) Image Acquisition and Analysis
- 07:48Results: Exosome Quantification by Analysis of LMDFM Images
- 08:25Conclusion
Clinical translation of exosome-derived biomarkers for diseased and malignant cells is hindered by the lack of rapid and accurate quantification methods. This report describes the use of low-magnification dark-field microscope images to quantify specific exosome subtypes in small volume serum or plasma samples.
