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Genetics

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Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
 

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Article doi: 10.3791/59233
March 1st, 2019

Summary March 1st, 2019

Traditional cDNA-based overexpression techniques have a limited applicability for the overexpression of long noncoding RNAs due to their multiple splice forms with potential functionality. This review reports a protocol using CRISPR technology to overexpress multiple splice variants of a long noncoding RNA.

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