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JoVE Journal
Genetics

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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
 

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Article DOI: 10.3791/59235-v
March 29th, 2019

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Summary March 29th, 2019

This protocol outlines the steps needed to generate a model system in which the transcription of an endogenous gene of interest can be conditionally controlled in live animals or cells using enhanced lac repressor and/or tet activator systems.

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