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Cancer Research
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体外肿瘤细胞恢复对 chimeric 抗原受体 t 细胞抗肿瘤功能的预测评价
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
Please note that all translations are automatically generated.
Click here for the English version.
体外肿瘤细胞恢复对 chimeric 抗原受体 t 细胞抗肿瘤功能的预测评价
DOI:
10.3791/59275-v
•
08:04 min
•
February 27, 2019
•
Dongrui Wang
2
,
Renate Starr
,
Darya Alizadeh
,
Xin Yang
,
Stephen J. Forman*
1
,
Christine E. Brown*
1
1
Department of Hematology & Hematopoietic Cell Transplantation, T Cell Therapeutics Research Laboratory
,
City of Hope Beckman Research Institute and Medical Center
,
2
Irell and Manella Graduate School of Biological Sciences
,
City of Hope Beckman Research Institute and Medical Center
Chapters
00:04
Title
00:54
Tumor Cell: T Cell Co-culture Setup and Tumor Cell Rechallenge
02:37
Sample Harvest for Flow Cytometric Analysis
04:12
Functional and Phenotypic Chimeric Antigen Receptor (CAR) T Cell Analysis
05:14
Results: Representative CD4+ and CD8+ CAR T Cell Phenotypes After Repetitive Challenge
07:30
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们描述了一种体外共培养方法, 递归挑战肿瘤靶向 t 细胞, 允许表型和功能分析抗肿瘤 t 细胞活性。
Tags
In Vitro
Tumor Cell
Rechallenge
Chimeric Antigen Receptor
CAR T Cells
Anti-tumor Function
Glioblastoma
Tumorspheres
Co-culture
High Throughput Screening
Cell Dissociation
Cell Suspension
Cell Culture
Supernatant
Trypsin EDTA
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