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利用 dna 折纸模板将金纳米产物组装成手性质浆细胞分裂体

JoVE Journal
Chemistry

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JoVE Journal Chemistry

Assembly of Gold Nanorods into Chiral Plasmonic Metamolecules Using DNA Origami Templates

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利用 dna 折纸模板将金纳米产物组装成手性质浆细胞分裂体

DOI:

10.3791/59280-v

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09:17 min

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March 05, 2019

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Yike Huang, Minh-Kha Nguyen², Anton Kuzyk

¹Department of Neuroscience and Biomedical Engineering,Aalto University School of Science, Aalto FI-00076, ²Faculty of Chemical Engineering, Ho Chi Minh City (HCMC) University of Technology,Vietnam National University – Ho Chi Minh City (VNU-HCM), Ho Chi Minh City 700000

Chapters

00:04Title
00:33Design of the DNA Origami and Assembly of the DNA Origami Templates
01:45DNA Origami Purification
03:03Synthesis of Gold Nanorods
04:52Functionalization of Gold Nanorods with Single-Stranded DNA
06:09Assembly of Gold Nanorods on DNA Origami Templates and Transmission Electron Microscopy (TEM) Imaging
07:45Results: TEM images of DNA Origami Templates, Gold Nanorods, and Final Chiral Plasmonic Metamolecules
08:43Conclusion

Summary

Automatic Translation

我们描述了金纳米棒 dna 起源组装成手性质粒元泡的详细协议, 具有较强的细胞反应。该协议不限于手性配置, 可以很容易地适应各种等离子体结构的制造。

Tags

Keywords: Gold Nanorods Chiral Plasmonic Metamolecules DNA Origami Templates Chiroptical Response CaDNAno Scaffold Strands Staple Strands Polyadenine Handles Lock Sequences Origami Annealing Agarose Gel Electrophoresis DNA Stain Origami Band Extraction Gold Nanorod Preparation

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