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Chapters
- 00:04Title
- 02:10Cell Treatments and Laser Setup
- 03:44Image Acquisition Setup and Collection
- 05:54Data Analysis
- 08:08Data Analysis in a Spreadsheet Program
- 10:16Results: Fluorescence Recovery After Photobleaching of Yellow Fluorescent Protein Tagged p62 in Aggresome-like Induced Structures
- 11:20Conclusion
We describe a comprehensive and practical protocol for fluorescence recovery after photobleaching experiments with live cells. Although the protocol was used to measure the mobility of yellow fluorescent protein-tagged p62 in aggresome-like induced structures, it can be applied to a variety of microscopy systems and fluorescent proteins.
