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Purification of the Dendritic Filopodia-rich Fraction

Purification of the Dendritic Filopodia-rich Fraction

Article doi: 10.3791/59292
May 2nd, 2019

Summary May 2nd, 2019

In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.


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