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Purification of the Dendritic Filopodia-rich Fraction

Article DOI: 10.3791/59292-v • 11:51 min • May 2nd, 2019
May 2nd, 2019 •
1,2, 3
1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2Liver Cancer Prevention Research Unit, RIKEN Center for Integrative Medical Sciences, 3Laboratory for Systems Molecular Ethology, RIKEN Center for Brain Science

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Summary
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In this protocol, we introduce a method for purifying the dendritic filopodia-rich fraction from the phagocytic cup-like protrusion structure on cultured hippocampal neurons by taking advantage of the specific and strong affinity between a dendritic filopodial adhesion molecule, TLCN, and an extracellular matrix molecule, vitronectin.

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Dendritic Filopodia-rich Fraction Synaptic Protein Immature Synapse Live Neuron Phagocytic Cap Formation Artificial Genesis Vitamin Mix Vitamin B5 Choline Chloride Folic Acid I-inositol Vitamin B3 Vitamin B6 Hydrochloride Thiamine Hydrochloride Riboflavin Solution Calcium Dichloride Minimum Essential Medium Potassium Chloride Sodium Chloride Sodium Bicarbonate Sodium Phosphate Monobasic Dihydrate D-glucose Magnesium Sulfate Heptahydrate
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