Journal
/
Biochemistry
/
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

DOI:
10.3791/59308-v

•

08:37 min

•

June 25, 2019

•

, , , ,
1Department of Sciences and Technologies (DST),University of Sannio, 2Endocannabinoid Research Group,Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions,University of Naples Federico II

Chapters

  • 00:04Title
  • 01:13Tissue Cutting
  • 01:45Blocking of Nonspecific Binding Sites and Tissue Permeabilization
  • 02:44Incubation with Antibodies
  • 03:18Tissue Mounting
  • 03:56Controls
  • 04:26Image Acquisition
  • 05:31Results: Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here are protocols for immunohistochemical characterization and localization of orexin peptide, orexin receptors, and endocannabinoid receptors in the gut and brains of normal and diet-induced obesity (DIO) adult zebrafish models using immunoperixidase and double immunofluorescence methods.

Tags

Keywords: ImmunohistochemistryImmunoperoxidaseImmunofluorescenceZebrafishOrexinEndocannabinoid ReceptorsCryosectioningBlockingPrimary AntibodiesIncubation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59308?article_type=v

Identification of Fatty Acids in Bacillus cereus
check_url/59308?article_type=v

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
check_url/59308?article_type=v

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
check_url/59308?article_type=v

The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
check_url/59308?article_type=v

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
check_url/59308?article_type=v

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach
check_url/59308?article_type=v

Nitropeptide Profiling and Identification Illustrated by Angiotensin II
check_url/59308?article_type=v

SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
check_url/59308?article_type=v

Profiling Ubiquitin and Ubiquitin-like Dependent Post-translational Modifications and Identification of Significant Alterations
check_url/59308?article_type=v

Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn’s Disease using Immunofluorescence

Read Article