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Identifikation af orexin og Endocannabinoide receptorer hos voksne zebrafisk ved hjælp af Immunoperoxidase og immunofluorescens metoder
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
DOI:

08:37 min

June 25, 2019

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Chapters

  • 00:04Title
  • 01:13Tissue Cutting
  • 01:45Blocking of Nonspecific Binding Sites and Tissue Permeabilization
  • 02:44Incubation with Antibodies
  • 03:18Tissue Mounting
  • 03:56Controls
  • 04:26Image Acquisition
  • 05:31Results: Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish
  • 07:18Conclusion

Summary

Automatic Translation

Præsenteret her er protokoller for immunhistokemiske karakterisering og lokalisering af orexin peptid, orexin receptorer, og endocannabinoide receptorer i tarmen og hjernen af normale og kost-induceret fedme (Dio) voksne: zebrafisk modeller ved hjælp af immunoperixidase og dobbelte immunofluorescens metoder.

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