11,317 Views
•
07:33 min
•
May 01, 2019
DOI:
Summary
Automatically generated
This protocol allows rapid and efficient conversion of induced pluripotent stem cells into motor neurons with a spinal or cranial identity, by ectopic expression of transcription factors from inducible piggyBac vectors.
Related Videos
08:24
The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
Related Videos
6962 Views
10:02
piggyBac Transposon System Modification of Primary Human T Cells
Related Videos
17291 Views
06:01
In vitro Neuromuscular Junction Induced from Human Induced Pluripotent Stem Cells
Related Videos
5757 Views
N/A
Differentiation of Porcine Induced Pluripotent Stem Cells (piPSCs) into Neural Progenitor Cells (NPCs)
Related Videos
866 Views
13:58
Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
Related Videos
15492 Views
09:42
Efficient Differentiation of Mouse Embryonic Stem Cells into Motor Neurons
Related Videos
22349 Views
09:34
Automated Production of Human Induced Pluripotent Stem Cell-Derived Cortical and Dopaminergic Neurons with Integrated Live-Cell Monitoring
Related Videos
7217 Views
02:09
PiggyBac Transposon-Mediated Gene Editing in Human iPSCs: A Procedure to Integrate Gene of Interest in Human iPSCs Using PiggyBac Transposon System
Related Videos
1340 Views
01:35
In Vitro Induction of Neuromuscular Junctions from Engineered Human-Induced Pluripotent Stem Cells
Related Videos
96 Views
01:27
Human Pluripotent Stem Cell Differentiation in Neurons Using Engineered Lentiviruses
Related Videos
116 Views
Read Article
Cite this Article
Garone, M. G., de Turris, V., Soloperto, A., Brighi, C., De Santis, R., Pagani, F., Di Angelantonio, S., Rosa, A. Conversion of Human Induced Pluripotent Stem Cells (iPSCs) into Functional Spinal and Cranial Motor Neurons Using PiggyBac Vectors. J. Vis. Exp. (147), e59321, doi:10.3791/59321 (2019).
Copy